1α,25(OH)₂D₃ Promotes the Autophagy of Porcine Ovarian Granulosa Cells as a Protective Mechanism against ROS through the BNIP3/PINK1 Pathway.

ABSTRACT: Vitamin D (VD) is one of the important nutrients required by livestock; however, VD deficiency is reported to be widespread. Earlier studies have suggested a potential role for VD in reproduction. Studies on the correlation between VD and sow reproduction are limited. The aim of the current study was aimed to determine the role of 1,25-dihydroxy vitamin D₃ (1α,25(OH)₂D₃) on porcine ovarian granulosa cells (PGCs) in vitro to provide a theoretical basis for improving the reproductive efficiency of sows. We used chloroquine (autophagy inhibitor) and reactive oxygen species (ROS) scavenger N-acetylcysteine in conjunction with 1α,25(OH)₂D₃ to explore the effect on PGCs. The results showed that 10 nM of 1α,25(OH)₂D₃ inc

SUBMITTER: Wang S 

PROVIDER: S-EPMC10001661 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature