Conserved Transcriptome Features Define Prepubertal Primate Spermatogonial Stem Cells as A_dark Spermatogonia and Identify Unique Regulators.

ABSTRACT: Antineoplastic treatments for cancer and other non-malignant disorders can result in long-term or permanent male infertility by ablating spermatogonial stem cells (SSCs). SSC transplantation using testicular tissue harvested before a sterilizing treatment is a promising approach for restoring male fertility in these cases, but a lack of exclusive biomarkers to unequivocally identify prepubertal SSCs limits their therapeutic potential. To address this, we performed single-cell RNA-seq on testis cells from immature baboons and macaques and compared these cells with published data from prepubertal human testis cells and functionally-defined mouse SSCs. While we found discrete groups of human spermatogonia, baboon and rhesus spermatogonia appeared less heterogenous. A cross-species analysis re

SUBMITTER: Singh A 

PROVIDER: S-EPMC10002546 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature