Screening in serum-derived medium reveals differential response to compounds targeting metabolism.

ABSTRACT: A challenge for screening new candidate drugs to treat cancer is that efficacy in cell culture models is not always predictive of efficacy in patients. One limitation of standard cell culture is a reliance on non-physiological nutrient levels to propagate cells. Which nutrients are available can influence how cancer cells use metabolism to proliferate and impact sensitivity to some drugs, but a general assessment of how physiological nutrients affect cancer cell response to small molecule therapies is lacking. To enable screening of compounds to determine how the nutrient environment impacts drug efficacy, we developed a serum-derived culture medium that supports the proliferation of diverse cancer cell lines and is amenable to high-throughput screening. We used this system to screen sever

SUBMITTER: Abbott KL 

PROVIDER: S-EPMC10002634 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature