Temperature modulates the osmosensitivity of tilapia prolactin cells.

ABSTRACT: In euryhaline fish, prolactin (Prl) plays an essential role in freshwater (FW) acclimation. In the euryhaline and eurythermal Mozambique tilapia, Oreochromis mossambicus, Prl cells are model osmoreceptors, recently described to be thermosensitive. To investigate the effects of temperature on osmoreception, we incubated Prl cells of tilapia acclimated to either FW or seawater (SW) in different temperature (20, 26 and 32°C) and osmolality (280, 330 and 420 mOsm/kg) combinations for 6 h. Release of both Prl isoforms, Prl₁₈₈ and Prl₁₇₇, increased in hyposmotic media and were further augmented with a rise in temperature. Hyposmotically-induced release of Prl₁₈₈ was inhibited at 20°C. In SW fish, mRNA expression of prl₁₈₈ and prl₁

SUBMITTER: Hewage TMG 

PROVIDER: S-EPMC10002831 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature