Aquaporin 5 maintains lens transparency by regulating the lysosomal pathway using circRNA.

ABSTRACT: The lens is transparent, non-vascular, elastic and wrapped in a transparent capsule. The lens oppacity of AQP5^-/- mice was increased more than that of wild-type (AQP5^+/+ ) mice. In this study, we explored the potential functional role of circular RNAs (circRNAs) and transcription factor HSF4 in lens opacity in aquaporin 5 (AQP5) knockout (AQP5^-/- ) mice. Autophagy was impaired in the lens tissues of AQP5^-/- mice. Autophagic lysosomes in lens epithelial cells of AQP5^-/- mice were increased compared with AQP5^+/+ mice, based on analysis by transmission electron microscopy. The genetic information of the mice lens was obtained by high-throughput sequencing, and then the downstream genes were analysed. A circRNA-miRNA-mRNA networ

SUBMITTER: Shaohua H 

PROVIDER: S-EPMC10002928 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature