Advancing proficiency testing for ultra in resource-limited settings using dried tube specimen: A study by SRL-Uganda.

ABSTRACT:

Background

Proficiency testing (PT) has been hard to set up due to cost limitations and technical capacity. Conventional Xpert MTB/RIF PT programs use liquid and culture spots which require stringent storage and transportation conditions with cross-contamination chances prevalent. These setbacks prompted the use of dried tube specimens (DTS) for Ultra assay PT. For continuity of PT provision, stability of DTS and compatibility with testing protocols when kept for a long period needs to be established.

Methods

DTS were prepared from known isolates inactivated using a hot air oven at 85°C. 100μl of bacterial suspensions were aliquoted and dried inside a Biosafety cabinet. Panel validation was done to establish the baseline Deoxyribonucleic acid (DNA) concentration in terms of

SUBMITTER: Kabugo J 

PROVIDER: S-EPMC10004543 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature