H-rGO-Pd NPs Nanozyme Enhanced Silver Deposition Strategy for Electrochemical Detection of Glypican-3.

ABSTRACT: Glypican-3 (GPC3), as an emerging biomarker, has been shown to be beneficial for the early diagnosis and treatment of hepatocellular carcinoma (HCC). In this study, an ultrasensitive electrochemical biosensor for GPC3 detection has been constructed based on the hemin-reduced graphene oxide-palladium nanoparticles (H-rGO-Pd NPs) nanozyme-enhanced silver deposition signal amplification strategy. When GPC3 specifically interacted with GPC3 antibody (GPC3_Ab) and GPC3 aptamer (GPC3_Apt), an "H-rGO-Pd NPs-GPC3_Apt/GPC3/GPC3_Ab" sandwich complex was formed with peroxidase-like properties which enhanced H₂O₂ to reduce the silver (Ag) ions in solution to metallic Ag, resulting in the deposition of silver nanoparticles (Ag NPs) on the su

SUBMITTER: Li G 

PROVIDER: S-EPMC10004744 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature