Characterization of peptide O⋯HN hydrogen bonds via¹H-detected ¹⁵N/¹⁷O solid-state NMR spectroscopy.

ABSTRACT: High sensitivity and resolution solid-state NMR methods are reported, that straightforwardly select hydrogen-bonded ¹⁵N-¹⁷O pairs from amongst all other nitrogen and oxygen sites in peptides, to aid protein secondary and tertiary structure determination. Significantly improved sensitivity is obtained with indirect ¹H detection under fast MAS and stronger relayed dipole couplings.

SUBMITTER: Hung I 

PROVIDER: S-EPMC10004979 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature