High-resolution and high-speed 3D tracking of microrobots using a fluorescent light field microscope.

ABSTRACT:

Background

Imaging and tracking are crucial for microrobots which navigate through complex 3D environments. Fluorescent imaging (FI) by microscope offers a high-resolution and high-sensitive imaging method to study the property of microrobots. However, conventional microscope suffers from shallow depth of field (DOF) and lacks 3D imaging capability.

Methods

We proposed a high-resolution and high-speed 3D tracking method for microrobots based on a fluorescent light field microscope (FLFM). We designed the FLFM system according to the size of a representative helical microrobot (150 μm body length, 50 μm screw diameter), and studied the system's performance. We also proposed a 3D tracking algorithm for microrobots using digital refocusing.

Results

We validated the metho

SUBMITTER: Lv J 

PROVIDER: S-EPMC10006150 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature