PTBP1 drives c-Myc-dependent gastric cancer progression and stemness.

ABSTRACT:

Background

Gastric cancer (GC) tumorigenesis and treatment failure are caused by cancer stem cells. Polypyrimidine tract binding protein 1 (PTBP1) was shown to be involved in the development of embryonic stem cells and is now being considered as a therapeutic target for tumour progression and stem-cell characteristics.

Methods

PTBP1 expression in GC samples was detected using tissue microarrays. Proliferation, colony formation, spheroid formation and stem-cell analysis were used to examine PTBP1's role in tumorigenesis and stem-cell maintenance. In AGS and HGC-27 cells with or without PTBP1 deficiency, ubiquitin-related protein expression and co-precipitation assays were performed.

Results

We identified that PTBP1 was aberrantly highly expressed and represented a nove

SUBMITTER: Ni T 

PROVIDER: S-EPMC10006230 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature