Hydrogel delivery of purinergic enzymes improves cardiac ischemia/reperfusion injury.

ABSTRACT:

Rationale

The innate immune response contributes to cardiac injury in myocardial ischemia/reperfusion (MI/R). Neutrophils are an important early part of the innate immune response to MI/R. Adenosine, an endogenous purine, is a known innate immune modulator and inhibitor of neutrophil activation. However, its delivery to the heart is limited by its short half-life (<30 s) and off-target side effects. CD39 and CD73 are anti-inflammatory homeostatic enzymes that can generate adenosine from phosphorylated adenosine substrate such as ATP released from injured tissue.

Objective

We hypothesize that hydrogel-delivered CD39 and CD73 target the local early innate immune response, reduce neutrophil activation, and preserve cardiac function in MI/R injury.

Methods and results

We

SUBMITTER: Sayegh MN 

PROVIDER: S-EPMC10006353 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature