ApoM binds endotoxin contributing to neutralization and clearance by High Density Lipoprotein.

ABSTRACT:

Background

HDL possesses anti-inflammatory properties, however, the exact mechanism is not fully understood. Endotoxin is a potent inducers of TLR4 signaling, leading to inflammatory mediators' release. It has been estimated that TLR4 recognizes about 5% of circulating lipopolysaccharide whereas 95% is cleared by plasma lipoproteins, mainly HDL. ApoM is required for HDL biogenesis and 95% of plasma ApoM is found associated with HDL, both are significantly reduced during sepsis.

Aim

The aim of this study is to investigate whether ApoM binds endotoxin and contributes to anti-inflammatory activity of HDL.

Methods

Isothermal Titration Calorimetry (ITC) was used to determine the binding of ultrapure E. coli LPS to the recombinant ApoM protein. Purified human HDL and

SUBMITTER: Mousa H 

PROVIDER: S-EPMC10006442 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature