Single-cell RNA sequencing identifies hippocampal microglial dysregulation in diet-induced obesity.

ABSTRACT: Obesity is a growing global concern in adults and youth with a parallel rise in associated complications, including cognitive impairment. Obesity induces brain inflammation and activates microglia, which contribute to cognitive impairment by aberrantly phagocytosing synaptic spines. Local and systemic signals, such as inflammatory cytokines and metabolites likely participate in obesity-induced microglial activation. However, the precise mechanisms mediating microglial activation during obesity remain incompletely understood. Herein, we leveraged our mouse model of high-fat diet (HFD)-induced obesity, which mirrors human obesity, and develops hippocampal-dependent cognitive impairment. We assessed hippocampal microglial activation by morphological and single-cell transcriptomic analysis to

SUBMITTER: Henn RE 

PROVIDER: S-EPMC10006681 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature