Bulk and mosaic deletions of Egfr reveal regionally defined gliogenesis in the developing mouse forebrain.

ABSTRACT: The epidermal growth factor receptor (EGFR) plays a role in cell proliferation and differentiation during healthy development and tumor growth; however, its requirement for brain development remains unclear. Here we used a conditional mouse allele for Egfr to examine its contributions to perinatal forebrain development at the tissue level. Subtractive bulk ventral and dorsal forebrain deletions of Egfr uncovered significant and permanent decreases in oligodendrogenesis and myelination in the cortex and corpus callosum. Additionally, an increase in astrogenesis or reactive astrocytes in effected regions was evident in response to cortical scarring. Sparse deletion using mosaic analysis with double markers (MADM) surprisingly revealed a regional requirement for EGFR in rostrodo

SUBMITTER: Zhang X 

PROVIDER: S-EPMC10006693 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature