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One-Pot Synthesis of Enzyme and Antibody/CaHPO₄ Nanoflowers for Magnetic Chemiluminescence Immunoassay of Salmonella enteritidis.

ABSTRACT: In this study, through a bioinspired strategy, the horseradish peroxidase (HRP) and antibody (Ab) were co-embedded into CaHPO₄ to prepare HRP-Ab-CaHPO₄ (HAC) bifunctional hybrid nanoflowers by one-pot mild coprecipitation. The as-prepared HAC hybrid nanoflowers then were utilized as the signal tag in a magnetic chemiluminescence immunoassay for application in the detection of Salmonella enteritidis (S. enteritidis). The proposed method exhibited excellent detection performance in the linear range of 10-10⁵ CFU/mL, with the limit of detection (LOD) of 10 CFU/mL. This study indicates great potential in the sensitive detection of foodborne pathogenic bacteria in milk with this new magnetic chemiluminescence biosensing platform.

SUBMITTER: Mao X

PROVIDER: S-EPMC10006971 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

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One-Pot Synthesis of Enzyme and Antibody/CaHPO4 Nanoflowers for Magnetic Chemiluminescence Immunoassay of Salmonella enteritidis.

Mao Xingchu X Ye Ranfeng R

Sensors (Basel, Switzerland) 20230303 5

In this study, through a bioinspired strategy, the horseradish peroxidase (HRP) and antibody (Ab) were co-embedded into CaHPO4 to prepare HRP-Ab-CaHPO4 (HAC) bifunctional hybrid nanoflowers by one-pot mild coprecipitation. The as-prepared HAC hybrid nanoflowers then were utilized as the signal tag in a magnetic chemiluminescence immunoassay for application in the detection of Salmonella enteritidis (S. enteritidis). The proposed method exhibited excellent dete ...[more]

PMID: 36904982

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