T-Cell Repertoire Analysis in the Conjunctiva of Murine Dry Eye Model.

ABSTRACT:

Purpose

Dry eye is closely related to the activation and proliferation of immune cells, especially T cells. However, the determination of the preferential T-cell clonotypes is technically challenging. This study aimed to investigate the characterization of T-cell receptor (TCR) repertoire in the conjunctiva during dry eye.

Methods

A desiccating stress animal model was established using C57/BL6 mice (8-10 weeks, female). After 7 days of stress stimulation, the slit-lamp image and Oregon-green-dextran staining were used to evaluate the ocular surface injury. Periodic acid-Schiff staining was used to measure the number of goblet cells. Flow cytometry was used to detect the activation and proliferation of T cells in the conjunctiva and cervical lymph nodes. Next-generation seque

SUBMITTER: Bao X 

PROVIDER: S-EPMC10007900 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature