A cybergenetic framework for engineering intein-mediated integral feedback control systems.

ABSTRACT: The ability of biological systems to tightly regulate targeted variables, despite external and internal disturbances, is known as Robust Perfect Adaptation (RPA). Achieved frequently through biomolecular integral feedback controllers at the cellular level, RPA has important implications for biotechnology and its various applications. In this study, we identify inteins as a versatile class of genetic components suitable for implementing these controllers and present a systematic approach for their design. We develop a theoretical foundation for screening intein-based RPA-achieving controllers and a simplified approach for modeling them. We then genetically engineer and test intein-based controllers using commonly used transcription factors in mammalian cells and demonstrate their exceptiona

SUBMITTER: Anastassov S 

PROVIDER: S-EPMC10008564 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature