Functional non-parametric mixed effects models for cytotoxicity assessment and clustering.

ABSTRACT: A multitude of natural and synthetic chemicals are present in our environment.Through the study of a compound's cytotoxicity, researchers can carefully set regulations regarding how much of a certain chemical in the ambient environment is tolerable. In the past, research has focused on point measurements such as the LD50. Instead, we consider entire time-dependent cellular response curves through the application of functional mixed effects models. We identify differences in such curves corresponding to the chemical's mode of action-i.e. how the compound attacks human cells. Through such analysis, we identify curve features to be used for cluster analysis via application of both k-means and self organizing maps. The data is analyzed by making use of functional principal components as a data

SUBMITTER: Ma T 

PROVIDER: S-EPMC10008646 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature