WT1⁺ glomerular parietal epithelial progenitors promote renal proximal tubule regeneration after severe acute kidney injury.

ABSTRACT: Rationale: Mammalian renal proximal tubules can partially regenerate after acute kidney injury (AKI). However, cells participating in the renal proximal tubule regeneration remain to be elucidated. Wilms' tumor 1 (WT1) expresses in a subtype of glomeruli parietal epithelial cells (PECs) in adult kidneys, it remains unclear whether these WT1⁺ PECs play a role in renal regeneration/repair after AKI. Methods: Ischemia-reperfusion injury (IRI) mouse model was used to investigate the expression pattern of WT1 in the kidney after severe AKI. Conditional deletion of WT1 gene mice were generated using Pax8^CreERT2 and WT1^fl/fl mice to examine the function of WT1. Then, genetic cell lineage tracing and single-cell RNA sequencing were performed to illust

SUBMITTER: Hong X 

PROVIDER: S-EPMC10008742 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature