Lactate exposure shapes the metabolic and transcriptomic profile of CD8+ T cells.

ABSTRACT:

Introduction

CD8+ T cells infiltrate virtually every tissue to find and destroy infected or mutated cells. They often traverse varying oxygen levels and nutrient-deprived microenvironments. High glycolytic activity in local tissues can result in significant exposure of cytotoxic T cells to the lactate metabolite. Lactate has been known to act as an immunosuppressor, at least in part due to its association with tissue acidosis.

Methods

To dissect the role of the lactate anion, independently of pH, we performed phenotypical and metabolic assays, high-throughput RNA sequencing, and mass spectrometry, on primary cultures of murine or human CD8+ T cells exposed to high doses of pH-neutral sodium lactate.

Results

The lactate anion is well tolerated by CD8+ T cells in pH neu

SUBMITTER: Barbieri L 

PROVIDER: S-EPMC10008868 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature