Hyperkinetic Rat Model Induced by Optogenetic Parafascicular Nucleus Stimulation.

ABSTRACT:

Objective

The parafascicular nucleus (PF) plays important roles in controlling the basal ganglia. It is not well known whether the PF affects the development of abnormal involuntary movements (AIMs). This study was aimed to find a role of the PF in development of AIMs using optogenetic methods in an animal model.

Methods

Fourteen rats were underwent stereotactic operation, in which they were injected with an adeno-associated virus with channelrhodopsin (AAV2-hSyn-ChR2-mCherry) to the lateral one third of the PF. Behavior test was performed with and without optical stimulation 14 days after the injection of the virus. AIM of rat was examined using AIM score. After the behavior test, rat's brain was carefully extracted and the section was examined using a fluorescence microsco

SUBMITTER: Chung M 

PROVIDER: S-EPMC10009241 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature