Preliminary analysis of hippocampus synaptic apoptosis and microglial phagocytosis induced by severe restraint stress.

ABSTRACT:

Aim

Several studies reported stress-induced microglial phagocytosis, but the biochemical mechanisms by which stress alters microglial synaptic phagocytosis are not fully uncovered. Local or limited apoptosis without cell death was observed at neuronal synapses in previous studies, and proposed as an upstream mechanism for microglial synapse elimination. In this micro-report, we aimed to preliminary examine local synaptic apoptosis in the mouse hippocampus following severe restraint stress, and its effect on microglial phagocytosis.

Methods

Mice were exposed to 10-day water immersion restraint stress (WIRS). Brain sections including stratum lucidum in the hippocampal CA3 subfield were stained with antibodies against cleaved caspase 3, ionized calcium-binding adapter molecule1

SUBMITTER: Enomoto S 

PROVIDER: S-EPMC10009418 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature