Dissecting operant alcohol self-administration using saccharin-fading procedure.

ABSTRACT:

Background

Although alcohol use disorder is a complex human pathology, the use of animal models represents an opportunity to study some aspects of this pathology. One of the most used paradigms to study the voluntary alcohol consumption in rodents is operant self-administration (OSA).

Aims

In order to facilitate the performance of this paradigm, we aim to describe some critical steps of OSA under a saccharin-fading procedure.

Material & methods

We used 40 male Wistar rats to study the process of acquiring the operant response through a saccharin-fading procedure under a fixed ratio (FR1) schedule of reinforcement. Next, we analyze the alcohol introduction and concentration increase, the effect of an alcohol deprivation, and the analogy between this paradigm with the D

SUBMITTER: Calleja-Conde J 

PROVIDER: S-EPMC10009421 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature