Rapid, visual and highly sensitive sexing of bovine embryos by recombinase polymerase amplification with CFI staining.

ABSTRACT: Early bovine embryo sexing both increases the number of offspring of the desired sex, and reduces the subsequent costs of processing unwanted offspring of the opposite sex. The need for cattle of different sexes varies from industry to industry, and a range of tools have been set up to meet this need, but most are energy- and time-consuming, hence it is important to establish a fast and convenient method for bovine embryo determination. Herein, we established a recombinase polymerase amplification (RPA) method combined with CFI dye (RPA-CFI) for sexing of bovine embryos. The assay is highly sensitive, specific, rapid and simple; it can be carried out in only 5 min at 37 °C in a metal bath, and results are visualised using a fluorescent colorimeter. Highly specific male-female common and ma

SUBMITTER: Pu X 

PROVIDER: S-EPMC10009442 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature