The K2: Open-source simultaneous triple-color TIRF microscope for live-cell and single-molecule imaging.

ABSTRACT: Imaging the dynamics and interactions of biomolecules at the single-molecule level in live cells and reconstituted systems has generated unprecedented knowledge about the biomolecular processes underlying many cellular functions. To achieve the speed and sensitivity needed to detect and follow individual molecules, these experiments typically require custom-built microscopes or custom modifications of commercial systems. The costs of such single-molecule microscopes, their technical complexity and the lack of open-source documentation on how to build custom setups therefore limit the accessibility of single-molecule imaging techniques. To advance the adaptation of dynamic single-molecule imaging by a wider community, we present the "K2": an open-source, simultaneous triple-color total inte

SUBMITTER: Niederauer C 

PROVIDER: S-EPMC10009532 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature