Dose Effect of Mesenchymal Stromal Cell Delivery Through Cardiopulmonary Bypass.

ABSTRACT:

Background

Neurologic impairments are a significant concern for survivors after pediatric cardiac surgery with cardiopulmonary bypass (CPB). We have previously shown that mesenchymal stromal cell (MSC) delivery through CPB has the potential to mitigate the effects of CPB on neural stem/progenitor cells. This study assessed the dose effects of MSCs.

Methods

Piglets (n = 20) were randomly assigned to 1 of 4 groups: control, CPB, or CPB followed by MSC administration with low and high doses (10 × 10⁶ and 100 × 10⁶ cells per kilogram). We assessed acute dose effect on cell distribution, multiorgan functions, systemic inflammation, microglia activation, and neural stem/progenitor cell activities.

Results

By magnetic resonance imaging, approximately 10

SUBMITTER: Kobayashi K 

PROVIDER: S-EPMC10009803 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature