The P323L substitution in the SARS-CoV-2 polymerase (NSP12) confers a selective advantage during infection.

ABSTRACT:

Background

The mutational landscape of SARS-CoV-2 varies at the dominant viral genome sequence and minor genomic variant population. During the COVID-19 pandemic, an early substitution in the genome was the D614G change in the spike protein, associated with an increase in transmissibility. Genomes with D614G are accompanied by a P323L substitution in the viral polymerase (NSP12). However, P323L is not thought to be under strong selective pressure.

Results

Investigation of P323L/D614G substitutions in the population shows rapid emergence during the containment phase and early surge phase during the first wave. These substitutions emerge from minor genomic variants which become dominant viral genome sequence. This is investigated in vivo and in vitro using SARS-CoV-2 with P323

SUBMITTER: Goldswain H 

PROVIDER: S-EPMC10009825 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature