Deep eutectic solvent-based manganese dioxide nanosheets composites for determination of DNA by a colorimetric method.

ABSTRACT:

Background

Nucleic acid is the carrier of genetic information and the keymolecule in life science. It is important to establish a simple and feasible method for nucleic acid quantification in complex biological samples.

Methods

Four kinds of hydrogen bond acceptors (choline chloride (ChCl), L-carnitine, tetrabutylammonium chloride (TBAC) and cetyltrimethylammonium bromide (CTAB)) were used to synthesize deep eutectic solvents (DESs) with hexafluoroisopropanol (HFIP). DESs based manganese dioxide (MnO2) nanosheets composites was synthesized and characterized. DNA concentration was determined by a UVVis spectrometer. The mechanism of DNA-DES/MnO2 colorimetric system was further discussed.

Results

The composite composed of DES/MnO2 exhibited excellent oxidase-like activi

SUBMITTER: Xu J 

PROVIDER: S-EPMC10010034 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature