Dataset Information

Transcriptomics explores the potential of flavonoid in non-medicinal parts of Saposhnikovia divaricata (Turcz.) Schischk.

ABSTRACT:

Introduction

Saposhnikovia divaricata is a traditional Chinese medicine in China, which is widely used in clinic. The root of S. divaricata is often used as medicine, but little research has been done on its other tissues.

Methods

In this study, the contents of root and leaf of S. divaricata were determined by HPLC, the differentially expressed genes were screened by transcriptome sequencing at molecular level, and then verified by network pharmacology.

Results

The results showed that the content of 4'-O-β-D-glucosyl-5-O-methylvisamminol in the leaves was significantly higher than that in the roots, which was about 3 times higher than that in the roots. In addition, 10 differentially expressed key enzyme genes were screened in plant hormone signal transduction, phenylpropanoid and flavonoid biosynthetic pathways. C4H and CYP98A were up-regulated in root, while F3H was down-regulated in root. They can be used as important candidate genes for the mechanism of quality difference of S. divaricata. Finally, network pharmacological validation showed that 5-O-methylvesamitol plays an important role in the treatment of ulcerative colitis.

Discussion

These findings not only provide insight into flavonoid biosynthesis in S. divaricata associated molecular regulation, but also provide a theoretical basis for the development and utilization of S. divaricata.

SUBMITTER: Chen Y

PROVIDER: S-EPMC10010146 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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Publications

Transcriptomics explores the potential of flavonoid in non-medicinal parts of <i>Saposhnikovia divaricata</i> (Turcz.) Schischk.

Chen Yuqiu Y Zhang Tao T Chen Changbao C Xu Zhefeng Z Liu Chunshuo C

Frontiers in plant science 20230227

<h4>Introduction</h4><i>Saposhnikovia divaricata</i> is a traditional Chinese medicine in China, which is widely used in clinic. The root of S. divaricata is often used as medicine, but little research has been done on its other tissues.<h4>Methods</h4>In this study, the contents of root and leaf of S. divaricata were determined by HPLC, the differentially expressed genes were screened by transcriptome sequencing at molecular level, and then verified by network pharmacology.<h4>Results</h4>The r ...[more]

PMID: 36923128

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Project description:The northeastern region of China experiences a distinctly cold climate influenced by the Siberian High during the winter months, thus resulting in severe cold weather conditions. Snow cover is prevalent and can persist for several months. This prolonged exposure to low temperatures necessitates specific adaptations in terms of agriculture and plant life, particularly for perennial herbs. Saposhnikovia divaricate (Turcz.) Schischk (SD) is a widely distributed perennial herb in the northeastern and northern provinces of China. However, there is limited documentation on the molecular mechanism through which this plant adapts to cold stress. Therefore, we elucidated the SD response to cold stress by transcriptome and metabolome analysis. Cold stress induced chlorosis and wilting in plants, thus leading to added function of antioxidant enzymes and higher levels of malondialdehyde, proline, soluble sugars. Notably, the differentially expressed genes (DEGs) were primarily related with sugar metabolism, ROS sweep, flavonoid and terpenoid biosynthesis, plant hormone signalling pathways, lipid metabolism, and transcription factors. Additionally, the differentially expressed metabolites (DEMs) mainly included lipids, flavonoids, terpenoid compounds, sugar-related metabolites, alkaloids and other metabolites. Furthermore, integrated analysis revealed coexpression patterns between carbohydrate metabolism-related genes and genes reference flavonoid and terpenoid biosynthesis, along with their corresponding metabolites. Finally, the qPCR results revealed notable over-expression levels of stress-related genes, including those participated in plant hormone signalling pathways (PP2C and AUX), flavonoid biosynthesis (CH3), antioxidant enzymes (AOX and CAT), and sugar-related metabolite metabolism (TPS, SPS, and SS). In conclusion, our findings suggest that cold stress strongly affects plant hormone signalling pathways, ROS scavenging mechanisms, unsaturated fatty acid synthesis and flavonoid and terpenoid biosynthesis in SD. These discoveries provide valuable insights into the impact of cold climates on herbaceous plants.