Anti-leukemic effect of menthol, a peppermint compound, on induction of apoptosis and autophagy.

ABSTRACT:

Background

Menthol, a natural compound in peppermint leaves, has several biological activities, including antioxidant, anti-inflammatory, antiviral, antibacterial and anticancer properties. This study revealed the anti-leukemic effects and its underlying mechanisms of the menthol related apoptosis signaling pathway and autophagy in both NB4 and Molt-4 leukemic cell lines.

Methods

Both leukemic cells were treated with menthol in various concentration. Cell viability was assessed using MTT assay, whereas apoptosis and autophagy were analyzed by flow cytometry using Annexin V-FITC/PI and anti-LC3/FITC antibodies staining, respectively. Apoptotic and autophagic related gene and protein expression were detected using RT-qPCR and western blot analysis, respectively. Moreover, STIT

SUBMITTER: Naksawat M 

PROVIDER: S-EPMC10010179 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature