Tissue-Specific Sex Difference in Mouse Eye and Brain Metabolome Under Fed and Fasted States.

ABSTRACT:

Purpose

Visual physiology and various ocular diseases demonstrate sexual dimorphisms; however, how sex influences metabolism in different eye tissues remains undetermined. This study aims to address common and tissue-specific sex differences in metabolism in the retina, RPE, lens, and brain under fed and fasted conditions.

Methods

After ad libitum fed or being deprived of food for 18 hours, mouse eye tissues (retina, RPE/choroid, and lens), brain, and plasma were harvested for targeted metabolomics. The data were analyzed with both partial least squares-discriminant analysis and volcano plot analysis.

Results

Among 133 metabolites that cover major metabolic pathways, we found 9 to 45 metabolites that are sex different in different tissues under the fed state and 6 to

SUBMITTER: Saravanan M 

PROVIDER: S-EPMC10010444 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature