AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain.

ABSTRACT: Enhancers are binding platforms for a diverse array of transcription factors that drive specific expression patterns of tissue- and cell-type-specific genes. Multiple means of assessing non-coding DNA and various chromatin states have proven useful in predicting the presence of enhancer sequences in the genome, but validating the activity of these sequences and finding the organs and developmental stages they are active in is a labor-intensive process. Recent advances in adeno-associated virus (AAV) vectors have enabled the widespread delivery of transgenes to mouse tissues, enabling in vivo enhancer testing without necessitating a transgenic animal. This protocol shows how a reporter construct that expresses EGFP under the control of a minimal promoter, which does not drive significant ex

SUBMITTER: Warren TL 

PROVIDER: S-EPMC10010840 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature