Mitochondrial haplotype modulates genome expression and mitochondrial structure/function in cardiomyocytes following volume overload.

ABSTRACT: Mitochondrial DNA (mtDNA) haplotype regulates mitochondrial structure/function and reactive oxygen species in aortocaval fistula (ACF) in mice. Here, we unravel the mitochondrial haplotype effects on cardiomyocyte mitochondrial ultrastructure and transcriptome response to ACF in vivo. Phenotypic responses and quantitative transmission electron microscopy (TEM) and RNA sequence at 3 days were determined after sham surgery or ACF in vivo in cardiomyocytes from wild-type (WT) C57BL/6J (C57ⁿ:C57^mt) and C3H/HeN (C3Hⁿ:C3H^mt) and mitochondrial nuclear exchange mice (C57ⁿ:C3H^mt or C3Hⁿ:C57^mt). Quantitative TEM of cardiomyocyte mitochondria C3HWT hearts have more electron-dense compact mitochondrial cristae comp

SUBMITTER: Guichard JL 

PROVIDER: S-EPMC10010923 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature