Basal re-esterification finetunes mitochondrial fatty acid utilization.

ABSTRACT:

Objective

Emerging evidence suggest the existence of constant basal lipolysis and re-esterification of a substantial fraction of thus liberated fatty acids. In stimulated lipolysis, the re-esterification is proposed to be a protective mechanism against lipotoxicity; however, the role of the lipolysis coupled to re-esterification under basal conditions has not been deciphered.

Methods

We used adipocytes (in vitro differentiated brown and white adipocytes derived from a cell line or primary SVF culture) to study the effect of inhibition of re-esterification by pharmacological DGAT1 and DGAT2 inhibitors alone or in combination. We then evaluated cellular energetics, lipolysis flux, and lipidomic parameters along with mitochondrial properties and fuel utilization.

Results

SUBMITTER: Sharma AK 

PROVIDER: S-EPMC10011057 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature