Mitochondrial respiration reduces exposure of the nucleus to oxygen.

ABSTRACT: The endosymbiotic theory posits that ancient eukaryotic cells engulfed O₂-consuming prokaryotes, which protected them against O₂ toxicity. Previous studies have shown that cells lacking cytochrome c oxidase (COX), required for respiration, have increased DNA damage and reduced proliferation, which could be improved by reducing O₂ exposure. With recently developed fluorescence lifetime microscopy (FLIM)-based probes demonstrating that the mitochondrial compartment has lower [O₂] than the cytosol, we hypothesized that the perinuclear distribution of mitochondria in cells may create a barrier for O₂ to access the nuclear core, potentially affecting cellular physiology and maintaining genomic integrity. To test this hypothesis, we utilized

SUBMITTER: Mori MP 

PROVIDER: S-EPMC10011062 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature