Characterization of a highly conserved MUC5B-degrading protease, MdpL, from Limosilactobacillus fermentum.

ABSTRACT: MUC5B is the predominant glycoprotein in saliva and is instrumental in the establishment and maintenance of multi-species eubiotic biofilms in the oral cavity. Investigations of the aciduric Lactobacillaceae family, and its role in biofilms emphasizes the diversity across different genera of the proteolytic systems involved in the nutritional utilization of mucins. We have characterized a protease from Limosilactobacillus fermentum, MdpL (Mucin degrading protease from Limosilactobacillus) with a high protein backbone similarity with commensals that exploit mucins for attachment and nutrition. MdpL was shown to be associated with the bacterial cell surface, in close proximity to MUC5B, which was sequentially degraded into low molecular weight fragments. Mapping the subs

SUBMITTER: Leo F 

PROVIDER: S-EPMC10011156 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature