High-performance glass filters for capturing and culturing circulating tumor cells and cancer-associated fibroblasts.

ABSTRACT: Various liquid biopsy methods have been developed for the non-invasive and early detection of diseases. In particular, the detection of circulating tumor cells (CTCs) and cancer-associated fibroblasts (CAFs) in blood has been receiving a great deal of attention. We have been developing systems and materials to facilitate such liquid biopsies. In this study, we further developed glass filters (with various patterns of holes, pitches, and non-adhesive coating) that can capture CTCs, but not white blood cells. We optimized the glass filters to capture CTCs, and demonstrated that they could be used to detect CTCs from lung cancer patients. We also used the optimized glass filters for detecting CAFs. Additionally, we further developed a system for visualizing the captured cells on the glass fil

SUBMITTER: Tanaka H 

PROVIDER: S-EPMC10011408 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature