Fiber enhancement and 3D orientation analysis in label-free two-photon fluorescence microscopy.

ABSTRACT: Fluorescence microscopy can be exploited for evaluating the brain's fiber architecture with unsurpassed spatial resolution in combination with different tissue preparation and staining protocols. Differently from state-of-the-art polarimetry-based neuroimaging modalities, the quantification of fiber tract orientations from fluorescence microscopy volume images entails the application of specific image processing techniques, such as Fourier or structure tensor analysis. These, however, may lead to unreliable outcomes as they do not isolate myelinated fibers from the surrounding tissue. In this work, we describe a novel image processing pipeline that enables the computation of accurate 3D fiber orientation maps from both grey and white matter regions, exploiting the selective multiscale enha

SUBMITTER: Sorelli M 

PROVIDER: S-EPMC10011555 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature