TRAK adaptors regulate the recruitment and activation of dynein and kinesin in mitochondrial transport.

ABSTRACT: Mitochondrial transport along microtubules is mediated by Miro1 and TRAK adaptors that recruit kinesin-1 and dynein-dynactin. To understand how these opposing motors are regulated during mitochondrial transport, we reconstitute the bidirectional transport of Miro1/TRAK along microtubules in vitro. We show that the coiled-coil domain of TRAK activates dynein-dynactin and enhances the motility of kinesin-1 activated by its cofactor MAP7. We find that TRAK adaptors that recruit both motors move towards kinesin-1's direction, whereas kinesin-1 is excluded from binding TRAK transported by dynein-dynactin, avoiding motor tug-of-war. We also test the predictions of the models that explain how mitochondrial transport stalls in regions with elevated Ca²⁺. Transport of Miro1/TRAK by kines

SUBMITTER: Canty JT 

PROVIDER: S-EPMC10011603 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature