Katnip is needed to maintain microtubule function and lysosomal delivery to autophagosomes and phagosomes.

ABSTRACT: The efficient delivery of lysosomes is essential for many cell functions, such as the degradation of unwanted intracellular components by autophagy and the killing and digestion of extracellular microbes within phagosomes. Using the amoeba Dictyostelium discoideum, we find that cells lacking Katnip (Katanin interacting protein) have a general defect in lysosomal delivery and although they make autophagosomes and phagosomes correctly, cells are then unable to digest them. Katnip is largely unstudied yet highly conserved across evolution. Previously studies found that Katnip mutations in animals cause defects in cilia structure. Here we show that Katnip plays a more general role in maintaining microtubule function. We find that loss of Katnip has no overall effect on microtubule dynam

SUBMITTER: Starling GP 

PROVIDER: S-EPMC10011725 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature