Cytotoxic CD161^-CD8⁺ T_EMRA cells contribute to the pathogenesis of systemic lupus erythematosus.

ABSTRACT:

Background

Systemic lupus erythematosus (SLE) is a prototypical autoimmune disease affecting multiple organs and tissues with high cellular heterogeneity. CD8⁺ T cell activity is involved in the SLE pathogenesis. However, the cellular heterogeneity and the underlying mechanisms of CD8⁺ T cells in SLE remain to be identified.

Methods

Single-cell RNA sequencing (scRNA-seq) of PBMCs from a SLE family pedigree (including 3 HCs and 2 SLE patients) was performed to identify the SLE-associated CD8⁺ T cell subsets. Flow cytometry analysis of a SLE cohort (including 23 HCs and 33 SLE patients), qPCR analysis of another SLE cohort (including 30 HCs and 25 SLE patients) and public scRNA-seq datasets of autoimmune diseases were employed to validate the f

SUBMITTER: Xiong H 

PROVIDER: S-EPMC10011749 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature