Direct surface analysis mass spectrometry uncovers the vertical distribution of cuticle-associated metabolites in plants.

ABSTRACT: The plant cuticle covers the plant's entire aerial surface and acts as the outermost protective layer. Despite being crucial for the survival of plants, surprisingly little is known about its biosynthesis. Conventional analytical techniques are limited to the isolation and depolymerization of the polyester cutin, which forms the cuticular scaffold. Although this approach allows the elucidation of incorporated cutin monomers, it neglects unincorporated metabolites participating in cutin polymerization. The feasibility of a novel approach is tested for in situ analysis of unpolymerized cuticular metabolites to enhance the understanding of cuticle biology. Intact cotyledons of Brassica napus and Arabidopsis thaliana seedlings are immersed in organic solvents for 60 second

SUBMITTER: Saladin S 

PROVIDER: S-EPMC10012332 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature