Attenuation of IFN signaling due to m⁶A modification of the host epitranscriptome promotes EBV lytic reactivation.

ABSTRACT:

Background

Reactivation of Epstein Barr virus (EBV) leads to modulation of the viral and cellular epitranscriptome. N6-methyladenosine (m⁶A) modification is a type of RNA modification that regulates metabolism of mRNAs. Previous reports demonstrated that m⁶A modification affects the stability and metabolism of EBV encoded mRNAs. However, the effect of reactivation on reprograming of the cellular mRNAs, and how this contributes to successful induction of lytic reactivation is not known.

Methods

Methylated RNA immunoprecipitation sequencing (MeRIP-seq), transcriptomic RNA sequencing (RNA-seq) and RNA pull-down PCR were used to screen and validate differentially methylated targets. Western blotting, quantitative real-time PCR (RT-qPCR) and immunocytochemis

SUBMITTER: Bose D 

PROVIDER: S-EPMC10012557 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature