Morphological and mitochondrial changes in murine choroid plexus epithelial cells during healthy aging.

ABSTRACT:

Background

Choroid plexuses (ChPs) are intraventricular structures mainly composed by specialized epithelial cells interconnected by tight junctions that establish the blood-cerebrospinal fluid (CSF) barrier. ChPs are essential to produce CSF and transport solutes from and into the brain. Deterioration of ChP function and morphology has been correlated to worsening of neurodegenerative disorders. We here map morpho-functional changes in the ChP epithelial cells during healthy aging, starting from young adult to 2-years old mice.

Methods

We used a multi-tiered approach, including transmission electron microscopy (TEM), immunohistochemistry, RT-qPCR, Western Blot and 2-photon microscopy (2-PM) at multiple timepoints ranging from young adult to 2-years old mice.

Results

SUBMITTER: Scarpetta V 

PROVIDER: S-EPMC10012601 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature