Core genome sequencing and genotyping of Leptospira interrogans in clinical samples by target capture sequencing.

ABSTRACT:

Background

The life-threatening pathogen Leptospira interrogans is the most common agent of leptospirosis, an emerging zoonotic disease. However, little is known about the strains that are currently circulating worldwide due to the fastidious nature of the bacteria and the difficulty to isolate cultures. In addition, the paucity of bacteria in blood and other clinical samples has proven to be a considerable challenge for directly genotyping the agent of leptospirosis directly from patient material. Our understanding of the genetic diversity of strains during human infection is therefore limited.

Methods

Here, we carried out hybridization capture followed by Illumina sequencing of the core genome directly from 20 clinical samples that were PCR positive for pathogenic Leptospi

SUBMITTER: Grillova L 

PROVIDER: S-EPMC10012794 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature