Bacterial flagellin is a dominant, stable innate immune activator in the gastrointestinal contents of mice and rats.

ABSTRACT: Intestinal contents comprise the largest repository of immunogenic ligands of microbial origin. We undertook this study to assess the predominant microbe-associated molecular patterns (MAMPs) present therein and the receptors) that mediate the innate immune responses to them. Here, we demonstrated that intestinal contents from conventional, but not germ-free, mice and rats triggered robust innate immune responses in vitro and in vivo. Such immune responses were abrogated in the absence of either myeloid differentiation factor 88 (MyD88) or Toll-like receptor (TLR) 5, but not TLR4, suggesting that the stimuli was flagellin (i.e., protein subunit of flagella that drives bacterial motility). Accordingly, pre-treating intestinal extracts with proteinase, thereby degrading

SUBMITTER: Vijay-Kumar M 

PROVIDER: S-EPMC10012918 | biostudies-literature | 2023 Jan-Dec

REPOSITORIES: biostudies-literature