Bioelectronic microfluidic wound healing: a platform for investigating direct current stimulation of injured cell collectives.

ABSTRACT: Upon cutaneous injury, the human body naturally forms an electric field (EF) that acts as a guidance cue for relevant cellular and tissue repair and reorganization. However, the direct current (DC) flow imparted by this EF can be impacted by a variety of diseases. This work delves into the impact of DC stimulation on both healthy and diabetic in vitro wound healing models of human keratinocytes, the most prevalent cell type of the skin. The culmination of non-metal electrode materials and prudent microfluidic design allowed us to create a compact bioelectronic platform to study the effects of different sustained (12 hours galvanostatic DC) EF configurations on wound closure dynamics. Specifically, we compared if electrotactically closing a wound's gap from one wound edge (i.e.

SUBMITTER: Shaner S 

PROVIDER: S-EPMC10013350 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature