Rab3 mediates a pathway for endocytic sorting and plasma membrane recycling of ordered microdomains.

ABSTRACT: The composition of the plasma membrane (PM) must be tightly controlled despite constant, rapid endocytosis, which requires active, selective recycling of endocytosed membrane components. For many proteins, the mechanisms, pathways, and determinants of this PM recycling remain unknown. We report that association with ordered, lipid-driven membrane microdomains (known as rafts) is sufficient for PM localization of a subset of transmembrane proteins and that abrogation of raft association disrupts their trafficking and leads to degradation in lysosomes. Using orthogonal, genetically encoded probes with tunable raft partitioning, we screened for the trafficking machinery required for efficient recycling of engineered microdomain-associated cargo from endosomes to the PM. Using this screen, we

SUBMITTER: Diaz-Rohrer B 

PROVIDER: S-EPMC10013782 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature