Detection and genetic characterization of canine kobuvirus from stray dogs in Shanghai, China.

ABSTRACT: In this study, rectal samples collected from 60 stray dogs in dog shelters were screened for canine kobuvirus and other enteroviruses by quantitative real-time reverse transcription polymerase chain reaction. Canine kobuvirus was detected in 25% (15/60) of the samples. In the 15 positive samples, the coinfection rates of canine distemper virus, canine coronavirus, canine astrovirus, canine norovirus, and canine rotavirus were 26.67%, 20.00%, 73.33%, 0%, and 20.00%, respectively. Phylogenetic analysis based on partial VP1 sequences identified a novel canine kobuvirus that was a recombinant of canine and feline kobuvirus. Bayesian evolutionary analysis revealed that the rate of evolution of the VP1 gene of canine kobuvirus was 1.36 × 10^-4 substitutions per site per year (95% highe

SUBMITTER: Deng B 

PROVIDER: S-EPMC10013983 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature