Dissecting the influence of cellular senescence on cell mechanics and extracellular matrix formation in vitro.

ABSTRACT: Tissue formation and healing both require cell proliferation and migration, but also extracellular matrix production and tensioning. In addition to restricting proliferation of damaged cells, increasing evidence suggests that cellular senescence also has distinct modulatory effects during wound healing and fibrosis. Yet, a direct role of senescent cells during tissue formation beyond paracrine signaling remains unknown. We here report how individual modules of the senescence program differentially influence cell mechanics and ECM expression with relevance for tissue formation. We compared DNA damage-mediated and DNA damage-independent senescence which was achieved through over-expression of either p16^Ink4a or p21^Cip1 cyclin-dependent kinase inhibitors in primary human

SUBMITTER: Brauer E 

PROVIDER: S-EPMC10014055 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature